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Chloroquine diphosphate ATM/ATR activateur

Name: Chloroquine diphosphate
Brand: Selleck Chemicals
SKU: S4157
Rating: 5 (211 reviews)

Réf. CatalogueS4157

Chloroquine diphosphate est un agent antipaludéen et antirhumatismal de type 4-aminoquinoléine, agissant également comme un activateur de l'ATM. La Chloroquine est également un inhibiteur des toll-like receptors (TLRs).

Chloroquine diphosphate ATM/ATR activateur Chemical Structure

Structure chimique

Poids moléculaire: 515.86

Aller à

Contrôle Qualité

Lot : Pureté : 99.97%

99.97

Cibles apparentées	PI3K Akt mTOR GSK-3 DNA-PK AMPK PDPK1 PTEN PP2A PDK
Autre ATM/ATR Inhibiteurs	Ceralasertib (AZD6738) AZD1390 Berzosertib (VE-822) Lartesertib (M4076) Camonsertib (RP-3500) KU-60019 KU-55933 VE-821 AZ20 AZD0156

Culture cellulaire, traitement et concentration de travail

Lignées cellulaires	Type dessai	Concentration	Temps dincubation	Description de lactivité	PMID
human K562 cells	Cytotoxic assay			Cytotoxicity against human K562 cells by MTT assay, IC50=31.83 nM	18538567
human KB cells	Cytotoxic assay			Cytotoxicity against human KB cells by microplate method, IC50=0.6 μM	14640524
human MDA-MB-468 cells	Cytotoxic assay		48 h	Cytotoxicity against human MDA-MB-468 cells after 48 hrs by SRB assay in presence of 3.5 uM 4-(2-chloro-10H-phenoxazin-10-yl)-N,N-diethylbutan-1-amine hydrochloride, GI50=2.81 μM	19945197
human MCF7 cells	Cytotoxic assay		48 h	Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay in presence of 0.4 uM 1-(1-(4-(7-phenyl-1H-imidazo[4,5-g]quinoxalin-6-yl)benzyl)piperidin-4-yl)-1H-benzo[d]imidazol-2(3H)-one, GI50=5.51 μM	19945197
human MDA-MB-231 cells	Cytotoxic assay		48 h	Cytotoxicity against human MDA-MB-231 cells after 48 hrs by SRB assay in presence of 3.5 uM 4-(2-chloro-10H-phenoxazin-10-yl)-N,N-diethylbutan-1-amine hydrochloride, GI50=6.08 μM	19945197
human HeLa cells	Cytotoxic assay			Cytotoxicity against human HeLa cells assessed as growth inhibition by MTT assay, IC50=30 μM	24354322
human HepG2 cells	Cytotoxic assay		48 h	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay, IC50=37.68 μM	23815186
mammary tumor FM3A	Cytotoxicity assay			Cytotoxicity of compound was tested against mouse mammary tumor FM3A cells, EC50 = 0.0000003 μM.	12570385
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against Plasmodium falciparum FcB1/Columbia infected in human red blood cells assessed as parasite growth inhibition after 48 hrs by [3H]-hypoxanthine incorporation assay, IC50 = 0.00011 μM.	25659770
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against chloroquine and pyrimethamine-resistant ring stage Plasmodium falciparum V1/S infected in human red blood cells after 48 hrs, IC50 = 0.0055 μM.	21207937
red blood cells	Antimalarial assay			Antimalarial activity against Plasmodium ovale ring stage infected in red blood cells in presence of AB+ human serum by drug susceptibility assay, IC50 = 0.0078 μM.	20937779
red blood cells	Antimalarial assay		48 hrs	Antimalarial activity against chloroquine-sensitive erythrocytic stage of Plasmodium falciparum NF54 infected in human red blood cells assessed as growth inhibition after 48 hrs by [3H]-hypoxanthine incorporation assay, IC50 = 0.0094 μM.	23685569
red blood cells	Antimalarial assay		48 hrs	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum D6 infected in human O positive red blood cells assessed as inhibition of [3H]-hypoxanthine uptake after 48 hrs, IC50 = 0.01 μM.	21155593
red blood cells	Antimalarial assay			Antimalarial activity against chloroquine-sensitive Plasmodium falciparum D6 infected in red blood cells after 72 hrs by Malstat reagent based LDH assay, IC50 = 0.011 μM.	28222317
red blood cells	Antimalarial assay		72 hrs	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum 3D7 infected in human type A-positive red blood cells assessed as growth inhibition after 72 hrs by spectrophotometrically, IC50 = 0.012 μM.	25311562
red blood cells	Antimalarial assay			Antimalarial activity against Plasmodium malariae ring stage infected in red blood cells in presence of AB+ human serum by drug susceptibility assay, IC50 = 0.014 μM.	20937779
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against chloroquine-sensitive, mefloquine-resistant ring stage Plasmodium falciparum D6 infected in human red blood cells after 48 hrs, IC50 = 0.0142 μM.	21207937
red blood cells	Antiplasmodial assay		72 hrs	Antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum D10 infected in human red blood cells after 72 hrs by parasite lactate dehydrogenase assay, IC50 = 0.0143 μM.	22917857
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against asexual erythrocyte stage of chloroquine-sensitive Plasmodium falciparum NF54 infected in human red blood cells after 48 hrs by lactate dehydrogenase assay, IC50 = 0.016 μM.	30296683
red blood cells	Antimalarial assay		96 hrs	Antimalarial activity against wild type early trophozoite stage of Plasmodium falciparum D6 in human red blood cells assessed as [3H]hypoxanthine uptake after 96 hrs, IC50 = 0.017 μM.	23448281
FM3A	Function assay			Selectivity for FM3A cells over Plasmodium falciparum as mean EC50 ratio, IC50 = 0.019 μM.	12408708
red blood cells	Antimalarial assay		48 hrs	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum 3D7 infected in human O positive red blood cells assessed as inhibition of [3H]-hypoxanthine uptake after 48 hrs, IC50 = 0.02 μM.	21155593
red blood cells	Antimalarial assay		72 hrs	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum D10 infected in human type A-positive red blood cells assessed as growth inhibition after 72 hrs by spectrophotometrically, IC50 = 0.02 μM.	25311562
red blood cells	Antimalarial assay			Antimalarial activity against ring stage Plasmodium falciparum 3D7 infected with human red blood cells incubated for 48 hrs by SYBR Green method, IC50 = 0.023 μM.	25725608
red blood cells	Antimalarial assay			Antimalarial activity against Plasmodium ovale trophozoite stage infected in red blood cells in presence of AB+ human serum by drug susceptibility assay, IC50 = 0.0232 μM.	20937779
red blood cells	Antimalarial assay			Antimalarial activity against Plasmodium falciparum 3D7 A infected in red blood cells assessed as inhibition of [3H]hypoxanthine incorporation after 24 hrs by scintillation counting, IC50 = 0.024 μM.	24900261
red blood cells	Antimalarial assay		48 hrs	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum Nigerian infected in human red blood cells after 48 hrs by [3H]-hypoxanthine incorporation assay, IC50 = 0.025 μM.	28800458
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum 3D7 infected in human red blood cells assessed as reduction in parasite growth after 48 hrs by Hoechst 33342 staining based flow cytometric analysis, IC50 = 0.026 μM.	30199706
HCT-8	Antimicrobial assay		48 hrs	Antimicrobial activity against Cryptosporidium parvum infected in human HCT-8 cells after 48 hrs by ELISA, IC50 = 0.027 μM.	18591280
red blood cells	Antimalarial assay		72 hrs	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum D10 infected in human type A-positive red blood cells assessed as parasite lactate dehydrogenase activity after 72 hrs by spectrophotometric analysis, IC50 = 0.029 μM.	22054038
red blood cells	Antimalarial assay		72 hrs	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum D10 infected in human A-positive red blood cells after 72 hrs by lactate dehydrogenase assay, IC50 = 0.03 μM.	21123074
red blood cells	Antimalarial assay			Antimalarial activity against chloroquine-sensitive Plasmodium falciparum 3D7 infected in red blood cells after 72 hrs by SYBR green 1 staining based fluorometric assay, IC50 = 0.03 μM.	27692831
3D7	Antiplasmodial assay			Antiplasmodial activity against Plasmodium falciparum 3D7 ring stage cells assessed as reduction parasitemia by Malstat assay, IC50 = 0.03 μM.	22858300
red blood cells	Antimalarial assay			Antimalarial activity against Plasmodium falciparum 3D7A infected in red blood cells assessed as inhibition of parasite growth after 24 hrs by [3H]hypoxanthine incorporation assay, IC50 = 0.036 μM.	25906200
red blood cells	Antimalarial assay			Antimalarial activity against chloroquine-sensitive Plasmodium falciparum D6 infected in human red blood cells assessed as reduction in parasitic LDH activity by Malstat reagent based spectrophotometric method, IC50 = 0.04 μM.	28800458
red blood cells	Antiplasmodial assay			Antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum D6 infected in red blood cells after 72 hrs by Malstat reagent based LDH assay, IC50 = 0.04 μM.	28315598
red blood cells	Antiplasmodial assay			Antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum D6 infected in red blood cells after 72 hrs by parasitic LDH assay, IC50 = 0.04 μM.	24900509
Vero	Antimalarial assay		72 hrs	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum D6 infected in African green monkey (Cercopithecus aethiops) Vero cells assessed as parasite LDH activity after 72 hrs, IC50 = 0.05 μM.	19833520
red blood cells	Antiplasmodial assay		4 hrs	Antiplasmodial activity against Plasmodium falciparum in human red blood cells after 4 hrs, IC50 = 0.055 μM.	17990865
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against chloroquine-sensitive ring stage Plasmodium falciparum 3D7 infected in human red blood cells after 48 hrs, IC50 = 0.089 μM.	21207937
B-cells	Function assay			Concentration required for half-maximal inhibition of CpG-ODN effect on thymidine uptake by WEHI 231 murine B-cells in the presence of alpha-sIgM., EC50 = 0.11 μM.	10406648
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against chloroquine and pyrimethamine resistant Plasmodium falciparum K1 infected in human red blood cells assessed as reduction in parasite viability after 48 hrs by [3H]-hypoxanthine incorporation assay, IC50 = 0.12 μM.	25746816
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against chloroquine/pyrimethamine-resistant Plasmodium falciparum K1 infected in human red blood cells assessed as [3H]-hypoxanthine incorporation incubated for 48 hrs prior to [3H]-hypoxanthine addition measured after 24 hrs by li, IC50 = 0.125 μM.	24268543
red blood cells	Antimalarial assay			Antimalarial activity against Plasmodium malariae trophozoite stage infected in red blood cells in presence of AB+ human serum by drug susceptibility assay, IC50 = 0.1279 μM.	20937779
red blood cells	Antimalarial assay		48 hrs	Antimalarial activity against chloroquine-resistant Plasmodium falciparum FcB1 infected in human red blood cells after 48 hrs by [3H]-hypoxanthine incorporation assay, IC50 = 0.132 μM.	28800458
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against chloroquine-resistant ring stage Plasmodium falciparum W2 infected in human red blood cells after 48 hrs, IC50 = 0.138 μM.	21207937
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against chloroquine-resistant Plasmodium falciparum W2 infected in human red blood cells assessed as inhibition of ring-stage parasite growth after 48 hrs by flow cytometry, IC50 = 0.14 μM.	21295887
RPMI1640	Antiparasitic assay		72 hrs	Antiparasitic activity against chloroquine-resistant erythrocytic stage of Plasmodium falciparum K1 infected in human RPMI1640 cells after 72 hrs by NBT staining based spectroscopic analysis, IC50 = 0.14 μM.	26922226
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against chloroquine/pyrimethamine resistant Plasmodium falciparum K1 infected in human red blood cells after 48 hrs by [3H]hypoxanthine incorporation assay, IC50 = 0.15 μM.	27344215
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against asexual erythrocyte stage of chloroquine-resistant Plasmodium falciparum Dd2 infected in human red blood cells after 48 hrs by lactate dehydrogenase assay, IC50 = 0.15 μM.	30296683
red blood cells	Antimalarial assay		48 hrs	Antimalarial activity against multidrug-resistant Plasmodium falciparum K1 infected in human red blood cells assessed as 3H-hypoxanthine incorporation after 48 hrs by liquid scintillation counting, IC50 = 0.17 μM.	21644541
L6	Antiparasitic assay		48 hrs	Antiparasitic activity against Plasmodium falciparum K1 infected in rat L6 cells assessed as inhibition of parasite growth preincubated for 48 hrs before [3H]hypoxanthine addition measured after 24 hrs by [3H]hypoxanthine incorporation assay, IC50 = 0.178 μM.	21937228
L6	Cytotoxicity assay			Cytotoxicity against rat L6 cells by alamar blue assay, IC50 = 0.1885 μM.	17178177
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against the multidrug-resistant Plasmodium falciparum FRC-3 infected in human red blood cells assessed as reduction in parasite growth after 48 hrs by Hoechst 33342 staining based flow cytometric analysis, IC50 = 0.207 μM.	30199706
red blood cells	Antimalarial assay		48 hrs	Antimalarial activity against chloroquine-resistant erythrocytic stage of Plasmodium falciparum K1 infected in human red blood cells assessed as growth inhibition after 48 hrs by [3H]-hypoxanthine incorporation assay, IC50 = 0.21 μM.	23685569
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against chloroquine- and pyrimethamine-resistant Plasmodium falciparum K1 infected in human red blood cells assessed as [3H]hypoxanthine incorporation after 48 hrs by liquid scintillation counting, IC50 = 0.22 μM.	21438586
red blood cells	Antimalarial assay			Antimalarial activity against ring stage Plasmodium falciparum W2 infected with human red blood cells incubated for 48 hrs by flow cytometry, IC50 = 0.229 μM.	25725608
L6	Antiparasitic assay			Antiparasitic activity against chloroquine, pyrimethamine-resistant Plasmodium falciparum K1 erythrocyte stage infected rat L6 cells, IC50 = 0.23 μM.	17949068
L6	Antiplasmodial assay			Antiplasmodial activity against chloroquine and pyrimethamine-resistant Plasmodium falciparum K1 infected in rat L6 cells, IC50 = 0.25 μM.	19942439
red blood cells	Antimalarial assay		72 hrs	Antimalarial activity against chloroquine-resistant Plasmodium falciparum W2 infected in human A-positive red blood cells after 72 hrs by lactate dehydrogenase assay, IC50 = 0.25 μM.	21123074
red blood cells	Antimalarial assay		48 hrs	Antimalarial activity against chloroquine-resistant Plasmodium falciparum FcM29 infected in human red blood cells after 48 hrs by [3H]-hypoxanthine incorporation assay, IC50 = 0.26 μM.	28800458
red blood cells	Antimalarial assay		48 hrs	Antimalarial activity against multidrug-resistant Plasmodium falciparum TM90-C2B infected in human O positive red blood cells assessed as inhibition of [3H]-hypoxanthine uptake after 48 hrs, IC50 = 0.28 μM.	21155593
red blood cells	Antimalarial assay		48 hrs	Antimalarial activity against ring stage Plasmodium falciparum Dd2 infected with human red blood cells incubated for 48 hrs by SYBR Green method, IC50 = 0.285 μM.	25725608
red blood cells	Antimalarial assay		96 hrs	Antimalarial activity against chloroquine and pyrimethamine-resistant early trophozoite stage of Plasmodium falciparum W2 in human red blood cells assessed as [3H]hypoxanthine uptake after 96 hrs, IC50 = 0.29 μM.	23448281
red blood cells	Antimalarial assay		72 hrs	Antimalarial activity against chloroquine-resistant Plasmodium falciparum W2 infected in human type A-positive red blood cells assessed as growth inhibition after 72 hrs by spectrophotometrically, IC50 = 0.316 μM.	25311562
red blood cells	Antimalarial assay		72 hrs	Antimalarial activity against chloroquine-resistant Plasmodium falciparum W2 infected in red blood cells after 72 hrs by Malstat reagent based LDH assay, IC50 = 0.317 μM.	28222317
red blood cells	Antiplasmodial assay		72 hrs	Antiplasmodial activity against chloroquine-resistant Plasmodium falciparum W2 infected in human red blood cells after 72 hrs by parasite lactate dehydrogenase assay, IC50 = 0.3171 μM.	22917857
red blood cells	Antiplasmodial assay		72 hrs	Antiplasmodial activity against chloroquine-resistant Plasmodium falciparum W2 infected in red blood cells after 72 hrs by parasitic LDH assay, IC50 = 0.39 μM.	24900509
red blood cells	Antimalarial assay		48 hrs	Antimalarial activity against chloroquine and pyrimethamine-resistant Plasmodium falciparum K1 infected in human O positive red blood cells assessed as inhibition of [3H]-hypoxanthine uptake after 48 hrs, IC50 = 0.4 μM.	21155593
red blood cells	Antimalarial assay			Antimalarial activity against chloroquine-resistant Plasmodium falciparum W2 infected in human red blood cells assessed as reduction in parasitic LDH activity by Malstat reagent based spectrophotometric method, IC50 = 0.42 μM.	28800458
red blood cells	Antiplasmodial assay		72 hrs	Antiplasmodial activity against chloroquine-resistant Plasmodium falciparum W2 infected in red blood cells after 72 hrs by Malstat reagent based LDH assay, IC50 = 0.43 μM.	28315598
Vero	Antimalarial assay		72 hrs	Antimalarial activity against chloroquine-resistant Plasmodium falciparum W2 infected in African green monkey (Cercopithecus aethiops) (Cercopithecus aethiops) Vero cells assessed as parasite LDH activity after 72 hrs, IC50 = 0.484 μM.	19833520
HEK293	Cytotoxicity assay		72 hrs	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay, IC50 = 0.49 μM.	29236492
red blood cells	Antimalarial assay		72 hrs	Antimalarial activity against chloroquine-resistant Plasmodium falciparum W2 infected in human type A-positive red blood cells assessed as parasite lactate dehydrogenase activity after 72 hrs by spectrophotometric analysis, IC50 = 0.52 μM.	22054038
red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against chloroquine-resistant Plasmodium falciparum W2 infected in human red blood cells after 48 hrs by SYBR test, IC50 = 0.59 μM.	26988303
red blood cells	Antimalarial assay		48 hrs	Antimalarial activity against multidrug-resistant Plasmodium falciparum TM93-C1088 infected in human O positive red blood cells assessed as inhibition of [3H]-hypoxanthine uptake after 48 hrs, IC50 = 0.62 μM.	21155593
Dd2	Antiplasmodial assay			Antiplasmodial activity against drug resistant Plasmodium falciparum Dd2 ring stage cells assessed as reduction parasitemia by Malstat assay, IC50 = 0.71 μM.	22858300
red blood cells	Antimalarial assay		72 hrs	Antimalarial activity against chloroquine-resistant Plasmodium falciparum W2 infected in red blood cells after 72 hrs by SYBR green 1 staining based fluorometric assay, IC50 = 0.75 μM.	27692831
red blood cells	Antimalarial assay		48 hrs	Antimalarial activity against chloroquine, pyrimethamine, and mefloquine-resistant Plasmodium falciparum Dd2 infected in human O positive red blood cells assessed as inhibition of [3H]-hypoxanthine uptake after 48 hrs, IC50 = 0.98 μM.	21155593
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells expressing CD81 assessed as cell viability after 72 hrs by CellTiter-glo assay, CC50 = 1.6 μM.	25791675
HepG2	Antiplasmodial assay		48 hrs	Antiplasmodial activity against liver stage of Plasmodium yoelii 17X NL sporozoites infected in human HepG2 cells expressing CD81 after 48 hrs by DAPI staining-based immunofluorescence analysis, IC50 = 1.65 μM.	25791675
WI38	Antiviral assay		2 hrs	Antiviral activity against BKV Gardner ATCC VR837 infected in human WI38 cells assessed as reduction in viral DNA level preincubated for 2 hrs before viral infection measured after 7 days by real time PCR assay, EC50 = 1.7 μM.	17893158
HeLa	Growth inhibition assay		48 hrs	Growth inhibition of human HeLa cells after 48 hrs by MTT assay in presence of 100 uM ZnCl2, GI50 = 2.1 μM.	19748786
SMB	Antiprion assay			Antiprion activity against Chandler strain of sheep scrapie prion protein infected in mouse SMB cells assessed as clearance of disease-associated scrapie prion protein, EC50 = 2.3 μM.	21570837
HEK293	Function assay			Inhibition of human ERG expressed in HEK293 cells assessed as reduction in tail current at membrane potential of +20 mV, IC50 = 2.5 μM.	25746816
red blood cells	Antimalarial assay		24 hrs	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum 3D7 infected in red blood cells assessed as reduction in mature schizonts formation after 24 hrs by Giemsa-staining based assay, EC50 = 2.97 μM.	29499486
HEK293	Function assay			hERG binding assays: Displacement of [3H]-Dofetilide (5 nM final) from hERG membranes obtained from HEK293 cells, Ki = 3.16228 μM.	32353859
1205 Lu	Cytotoxicity assay		72 hrs	Cytotoxicity against human 1205 Lu cells after 72 hrs by MTT assay, IC50 = 3.6 μM.	25699157
LN229	Cytotoxicity assay		72 hrs	Cytotoxicity against human LN229 cells after 72 hrs by MTT assay, IC50 = 3.6 μM.	25699157
C8161	Cytotoxicity assay		72 hrs	Cytotoxicity against human C8161 cells after 72 hrs by MTT assay, IC50 = 3.6 μM.	25699157
HT-29	Cytotoxicity assay		72 hrs	Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay, IC50 = 3.6 μM.	25699157
WI38	Cytotoxicity assay		24 hrs	Cytotoxicity against human WI38 cells assessed as inhibition of proliferation after 24 hrs by SRB assay, IC50 = 3.63 μM.	25461309
ScN2a	Function assay			Half maximal inhibition of Prion protein PrPsc formation was assayed in ScN2a cells, EC50 = 4 μM.	12904059
HepG2	Cytotoxicity assay		24 hrs	Cytotoxicity against human HepG2 cells after 24 hrs by neutral red dye uptake assay, TC50 = 4.3 μM.	22783984
HeLa	Growth inhibition assay		48 hrs	Growth inhibition of human HeLa cells after 48 hrs by MTT assay in presence of 50 uM CuCl2, GI50 = 4.4 μM.	19748786
HeLa	Growth inhibition assay		48 hrs	Growth inhibition of human HeLa cells after 48 hrs by MTT assay in presence of 10 uM CuCl2, GI50 = 5.3 μM.	19748786
HeLa	Growth inhibition assay		48 hrs	Growth inhibition of human HeLa cells after 48 hrs by MTT assay in presence of 50 uM ZnCl2, GI50 = 5.6 μM.	19748786
HeLa	Growth inhibition assay		48 hrs	Growth inhibition of human HeLa cells after 48 hrs by MTT assay in presence of 1 uM CuCl2, GI50 = 6.1 μM.	19748786
SH-SY5Y	Function assay		24 hrs	Modulation of human wild-type APP695 expressed in SH-SY5Y cells assessed as inhibition of amyloid beta (1 to 40 residues) production measured after 24 hrs, IC50 = 7 μM.	29559198
SH-SY5Y	Function assay		24 hrs	Inhibition of BACE1 in human SH-SY5Y cells harboring wild type APP695 assessed as reduction in amyloid beta (1 to 40) level after 24 hrs by ELISA method, IC50 = 7 μM.	30268822
CHO	Function assay			Inhibition of human ERG expressed in CHO cells, Ki = 7.5 μM.	24900603
L6	Cytotoxicity assay			Cytotoxicity against L6 cells, IC50 = 7.7 μM.	17280835
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells after 72 hrs by alamar blue assay, IC50 = 8.3 μM.	21155593
WI38	Cytotoxicity assay		2 hrs	Cytotoxicity against human WI38 cells assessed as reduction in cellular DNA level preincubated for 2 hrs before viral infection measured after 7 days by real time PCR assay, CC50 = 8.5 μM.	17893158
HepG2	Antimalarial assay			Antimalarial activity against sporozoite stage of Plasmodium berghei yoelii infected in human HepG2 cells, IC50 = 9 μM.	26640981
SKHep	Growth inhibition assay		48 hrs	Growth inhibition of human SKHep cells after 48 hrs by MTT assay in presence of 10% FBS supplemented DMEM medium, GI50 = 9.5 μM.	19748786
HeLa	Cytotoxicity assay		7 hrs	Cytotoxicity against human HeLa cells assessed as cell survival after 7 hrs by MTT assay, IC50 = 9.665 μM.	17768052
HeLa	Cytotoxicity assay		7 days	Cytotoxicity against human HeLa cells after 7 days by MTT assay, IC50 = 9.67 μM.	21489789
K562	Cytotoxicity assay		24 hrs	Cytotoxicity against human K562 cells assessed as radioactive thymidine incorporation after 24 hrs, CC50 = 9.7 μM.	18680278
Vero	Antiviral assay		6 to 7 days	Antiviral activity against chikungunya virus Indian ocean strain 899 infected in Vero cells assessed as virus-induced cytopathic effect after 6 to 7 days, EC50 = 10 μM.	25970561
PDAC	Function assay			Inhibition of cell proliferation of PDAC cells (unknown origin), IC50 = 10 μM.	26135471
Vero	Antiviral assay		6 to 7 days	Antiviral activity against Chikungunya virus 899 infected in African green monkey Vero cells assessed as inhibition of virus-induced cell death after 6 to 7 days by MTS assay, EC50 = 10 μM.	24926807
HepG2	Cytotoxicity assay		48 hrs	Cytotoxicity against human HepG2 cells after 48 hrs by CellTiter-Glo assay, EC50 = 10.1 μM.	29800827
BT-483	Growth inhibition assay		48 hrs	Growth inhibition of human BT-483 cells after 48 hrs by MTT assay in presence of 10% FBS supplemented DMEM medium, GI50 = 10.2 μM.	19748786
Vero	Antiviral assay		5 days	Antiviral activity against Chikungunya virus Indian Ocean 889 infected in African green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect after 5 days by MTS/PMS method, EC50 = 11 μM.	29336951
Vero A	Antiviral assay		6 to 7 days	Antiviral activity against Chikungunya virus Indian ocean strain 899 infected in African green monkey Vero A cells assessed as reduction in viral-induced cytopathic effect after 6 to 7 days by MTS/PMS assay, EC50 = 11 μM.	28671832
Vero	Antiviral assay		5 days	Antiviral activity against Chikungunya virus Indian Ocean strain 899 infected in African green monkey Vero cells assessed as inhibition of viral-induced cytopathic effect measured on day 5 post infection by MTS/PMS assay, EC50 = 11 μM.	29499487
Vero	Antiviral assay		7 days	Antiviral activity against Chikungunya virus Indian Ocean strain 899 infected in African green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect after 7 days by MTS/PMS method, EC50 = 11 μM.	24800626
Vero	Antiviral assay		7 days	Antiviral activity against Sindbis virus strain HRsp infected in african green monkey Vero cells assessed as inhibition of virus induced cell death measured after 7 days, EC50 = 11 μM.	25946116
Vero	Antiviral assay		7 days	Antiviral activity against Chikungunya virus strain 899 infected in african green monkey Vero cells assessed as inhibition of virus induced cell death measured after 7 days, EC50 = 11 μM.	25946116
Vero	Antiviral assay		6 to 7 days	Antiviral activity against Sindbis virus HRsp infected in African green monkey Vero cells assessed as inhibition of virus-induced cell death after 6 to 7 days by MTS assay, EC50 = 11 μM.	24926807
Vero	Antiviral assay		6 to 7 days	Antiviral activity against Sindbis virus HRsp infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect incubated for 6 to 7 days by MTS assay, EC50 = 11 μM.	23215460
Vero	Antiviral assay		6 to 7 days	Antiviral activity against Chikungunya virus 899 infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect incubated for 6 to 7 days by MTS assay, EC50 = 11 μM.	23215460
red blood cells	Antimalarial assay		48 hrs	Antimalarial activity against chloroquine-resistant Plasmodium falciparum K14 infected in human red blood cells after 48 hrs by [3H]hypoxanthine incorporation assay, IC50 = 11 μM.	25282260
red blood cells	Antimalarial assay		24 hrs	Antimalarial activity against chloroquine-resistant Plasmodium falciparum RKL9 infected in red blood cells assessed as reduction in mature schizonts formation after 24 hrs by Giemsa-staining based assay, EC50 = 11.25 μM.	29499486
MCF7	Cytotoxicity assay			Cytotoxicity against human MCF7 cells assessed as [3H]hypoxanthine incorporation, IC50 = 12 μM.	18512987
HEK293	Cytotoxicity assay		72 hrs	Cytotoxicity against human HEK293 cells after 72 hrs by alamar blue assay, IC50 = 12 μM.	21155593
Vero	Antiviral assay		24 hrs	Antiviral activity against Zika virus infected in African green monkey Vero cells assessed as inhibition of viral RNA replication after 24 hrs by RT-PCR method, EC50 = 12 μM.	28068604
Vero	Antiviral assay		24 hrs	Antiviral activity against Zika virus infected in African green monkey Vero cells assessed as inhibition of viral RNA replication after 24 hrs by RT-PCR method, EC50 = 12 μM.	28092859
HeLa	Antiviral assay		48 hrs	Antiviral activity against VSV-G pseudotyped HIV1 lentiviral particles infected in human HeLa cells incubated for 48 hrs by spectrofluorometry, IC50 = 12.48 μM.	22858300
SH-SY5Y	Function assay		24 hrs	Inhibition of BACE1 in human SH-SY5Y cells harboring wild type APP695 assessed as reduction in amyloid beta (1 to 42) level after 24 hrs by ELISA method, IC50 = 12.7 μM.	30268822
SH-SY5Y	Function assay		24 hrs	Modulation of human wild-type APP695 expressed in SH-SY5Y cells assessed as inhibition of amyloid beta (1 to 42 residues) production measured after 24 hrs by ELISA, IC50 = 12.8 μM.	29559198
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells after 72 hrs by Alamar Blue assay, IC50 = 12.9 μM.	21190857
Vero	Antiviral assay		6 to 7 days	Antiviral activity against Semliki forest virus Vietnam infected in African green monkey Vero cells assessed as inhibition of virus-induced cell death after 6 to 7 days by MTS assay, EC50 = 13.8 μM.	24926807
Vero	Antiviral assay		7 days	Antiviral activity against Semliki forest virus strain Vietnam infected in african green monkey Vero cells assessed as inhibition of virus induced cell death measured after 7 days, EC50 = 14 μM.	25946116
Vero	Antiviral assay		6 to 7 days	Antiviral activity against Semliki forest virus infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect incubated for 6 to 7 days by MTS assay, EC50 = 14 μM.	23215460
NSO	Cytotoxicity assay			Cytotoxicity against mouse NSO cells by MTT assay, TC50 = 14.9 μM.	19113955
L1210	Cytotoxicity assay			Cytotoxicity against mouse L1210 cells assessed as inhibition of cell proliferation by Coulter counter analysis, IC50 = 15 μM.	26114811
Huh-7	Antiplasmodial assay		48 hrs	Antiplasmodial activity against Plasmodium berghei liver stage form transfected in human Huh-7 cells assessed as cell viability after 48 hrs by luciferase reporter gene assay, IC50 = 15.9 μM.	23273038
Daudi	Cytotoxicity assay			Cytotoxicity against human Daudi cells by MTT assay, TC50 = 16.7 μM.	19113955
HeLa	Cytotoxicity assay			Cytotoxicity against human HeLa cells assessed as inhibition of cell proliferation by Coulter counter analysis, IC50 = 18 μM.	26114811
J774.1	Cytotoxicity assay		24 hrs	Cytotoxicity against mouse J774.1 cells after 24 hrs by AlamarBlue based cytotoxicity assay, IC50 = 18.4 μM.	27316542
HeLa	Growth inhibition assay		48 hrs	Growth inhibition of human HeLa cells after 48 hrs by MTT assay in presence of 10% FBS supplemented DMEM medium, GI50 = 18.6 μM.	19748786
MCF7	Cytotoxicity assay		48 hrs	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay, CC50 = 19.4 μM.	20014857
MCF7	Cytotoxicity assay		48 hrs	Cytotoxicity against human MCF7 cells after 48 hrs by XTT reduction assay, CC50 = 19.4 μM.	24686013
MCF7	Cytotoxicity assay		48 hrs	Cytotoxicity against human MCF7 cells assessed as decrease in cell viability after 48 hrs by XTT assay, CC50 = 19.4 μM.	24594524
MDA-MB-468	Antiproliferative assay		72 hrs	Antiproliferative activity against human MDA-MB-468 cells after 72 hrs by SRB assay, IC50 = 19.86 μM.	26602827
KB	Cytotoxicity assay		48 hrs	Cytotoxicity against human KB cells after 48 hrs by MTT assay, IC50 = 20.4 μM.	11809075
Vero	Antiviral assay		5 days	Antiviral activity against Chikungunya virus Indian Ocean 889 infected in African green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect after 5 days by MTS/PMS method, EC90 = 21 μM.	29336951
Vero	Antiviral assay			Antiviral activity against Chikungunya virus Indian Ocean strain 899 infected in African green monkey Vero cells assessed as inhibition of viral-induced cytopathic effect measured on day 5 post infection by MTS/PMS assay, EC90 = 21 μM.	29499487
Vero	Antiviral assay		7 days	Antiviral activity against Chikungunya virus Indian Ocean strain 899 infected in African green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect after 7 days by MTS/PMS method, EC90 = 21 μM.	24800626
Vero	Cytotoxicity assay		72 hrs	Cytotoxicity against african green monkey Vero cells assessed as growth inhibition after 72 hrs by MTS assay, IC50 = 21 μM.	23727538
HepG2	Cytotoxicity assay		48 hrs	Cytotoxicity against human HepG2 cells after 48 hrs by lactate dehydrogenase assay, GI50 = 22.16 μM.	21882831
KB	Cytotoxicity assay		72 hrs	Cytotoxicity against human KB cells after 72 hrs by MTS assay, IC50 = 22.5 μM.	19879150
MDA-MB-231	Cytotoxicity assay		48 hrs	Cytotoxicity against human MDA-MB-231 cells after 48 hrs by SRB assay, GI50 = 22.52 μM.	19804979
MDA-MB-231	Antiproliferative assay		48 hrs	Antiproliferative activity against human p53, pRB mutant and estrogen receptor deficient MDA-MB-231 cells after 48 hrs by SRB assay, GI50 = 22.52 μM.	20561720
MDA-MB-231	Cytotoxicity assay		48 hrs	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 48 hrs by SRB assay, IC50 = 22.52 μM.	28586716
CEM	Cytotoxicity assay			Cytotoxicity against human CEM cells assessed as inhibition of cell proliferation by Coulter counter analysis, IC50 = 23 μM.	26114811
CE81T	Growth inhibition assay		48 hrs	Growth inhibition of human CE81T cells after 48 hrs by MTT assay in presence of 10% FBS supplemented DMEM medium, GI50 = 23.4 μM.	19748786
MCF7	Cytotoxicity assay		72 hrs	Cytotoxicity against human MCF7 cells assessed as reduction in cell number incubated for 72 hrs, IC50 = 23.43 μM.	26135471
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells assessed as intracellular ATP level after 72 hrs by Celltiter-Glo luminescent assay, EC50 = 25 μM.	21910466
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells assessed as reduction in cell number incubated for 72 hrs, IC50 = 25 μM.	26135471
BxPC3	Antiproliferative assay		72 hrs	Antiproliferative activity against human BxPC3 cells after 72 hrs by SRB method, IC50 = 25 μM.	25699157
MDA-MB-468	Cytotoxicity assay		48 hrs	Cytotoxicity against human MDA-MB-468 cells after 48 hrs by SRB assay, GI50 = 28.58 μM.	19804979
MDA-MB-468	Cytotoxicity assay		48 hrs	Cytotoxicity against human MDA-MB-468 cells after 48 hrs by SRB assay, GI50 = 28.58 μM.	20106668
MDA-MB-468	Cytotoxicity assay		48 hrs	Cytotoxicity against human MDA-MB-468 cells assessed as growth inhibition after 48 hrs by SRB assay, IC50 = 28.58 μM.	28586716
K562	Cytotoxicity assay			Cytotoxicity against human K562 cells by MTT assay, IC50 = 29.03 μM.	18562202
HeLa	Antiproliferative assay		72 hrs	Antiproliferative activity against human HeLa cells after 72 hrs by SRB assay, IC50 = 29.96 μM.	26602827
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, IC50 = 30 μM.	19482476
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, IC50 = 30 μM.	19926173
HepG2	Cytotoxicity assay			Cytotoxicity against human HepG2 cells by MTT assay, IC50 = 30 μM.	17981462
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, CC50 = 30 μM.	21741131
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, CC50 = 30 μM.	22889559
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, CC50 = 30 μM.	24946216
SH-SY5Y	Cytotoxicity assay		72 hrs	Cytotoxicity against human SH-SY5Y cells after 72 hrs by MTT assay, CC50 = 30 μM.	29559198
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, CC50 = 30 μM.	24858543
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells measured after 72 hrs by MTT assay, CC50 = 30 μM.	27155463
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells assessed as cell viability after 72 hrs by MTT assay, CC50 = 30 μM.	25791675
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells incubated for 72 hrs by MTT assay, CC50 = 30 μM.	25282267
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, CC50 = 30 μM.	21852132
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, CC50 = 30 μM.	22608675
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay, CC50 = 30 μM.	27654395
KB	Cytotoxicity assay			Cytotoxicity against human KB cells by SRB assay, ED50 = 30.8 μM.	16562832
HepG2	Cytotoxicity assay		24 hrs	Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by neutral red uptake assay, TC50 = 31 μM.	23145816
K562	Cytotoxicity assay		72 hrs	Cytotoxicity against human K562 cells after 72 hrs by flow cytometry, IC50 = 32 μM.	19482476
K562	Cytotoxicity assay		72 hrs	Cytotoxicity against human K562 cells after 72 hrs by flow cytometry, IC50 = 32 μM.	19926173
FM3A	Antiproliferative assay			Antiproliferative activity against Mouse mammary tumor FM3A cells, EC50 = 32 μM.	10966746
FM3A	Cytotoxicity assay			In vitro cytotoxicity against mouse mammary FM3A cells, EC50 = 32 μM.	12036365
FM3A	Cytotoxicity assay			Cytotoxicity against mouse mammary tumor FM3A cells representing a model of host, EC50 = 32 μM.	11855978
FM3A	Cytotoxicity assay			Cytotoxicity against FM3A mouse mammary cells, EC50 = 32 μM.	13678413
FM3A	Cytotoxicity assay		48 hrs	Cytotoxicity against mouse FM3A cells assessed as inhibition of increase in cell density after 48 hrs, EC50 = 32 μM.	18479926
FM3A	Cytotoxicity assay			Compound was tested for cytotoxicity against FM3A mouse mammary cells. , EC50 = 32 μM.	10447961
LNCAP	Cytotoxicity assay		72 hrs	Cytotoxicity against human LNCAP cells assessed as reduction in cell number incubated for 72 hrs, IC50 = 32.87 μM.	26135471
HCT116	Cytotoxicity assay		72 hrs	Cytotoxicity against human HCT116 cells after 72 hrs by WST1 assay, IC50 = 33.7 μM.	12398531
KB	Cytotoxicity assay		72 hrs	Cytotoxicity against human KB cells after 72 hrs by SRB assay, ED50 = 33.7 μM.	9917283
KB	Cytotoxicity assay		72 hrs	Cytotoxicity against human KB cells after 72 hrs by Alamar blue assay, IC50 = 34 μM.	24900724
CHO	Cytotoxicity assay			Cytotoxicity against CHO cells by MTT assay, IC50 = 35.8 μM.	26832222
KB	Cytotoxicity assay			Cytotoxicity against human KB cells assessed as growth inhibition, IC50 = 35.95 μM.	21705220
MCF7	Cytotoxicity assay		48 hrs	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay, IC50 = 36.1 μM.	19892554
MDA-MB-231	Antiproliferative assay		72 hrs	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by SRB assay, IC50 = 36.53 μM.	26602827
NFF	Cytotoxicity assay		3 days	Cytotoxicity against human NFF cells after 3 days by sulforhodamine B assay, IC50 = 37.3 μM.	23117170
MCF7	Cytotoxicity assay		48 hrs	Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay, GI50 = 38.44 μM.	19804979
MCF7	Cytotoxicity assay		48 hrs	Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay, GI50 = 38.44 μM.	20106668
p53+/- MCF7	Antiproliferative assay		48 hrs	Antiproliferative activity against human p53+/- MCF7 cells after 48 hrs by SRB assay, GI50 = 38.44 μM.	20561720
MCF7	Cytotoxicity assay		48 hrs	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 48 hrs by SRB assay, IC50 = 38.44 μM.	28586716
Caco2	Antiproliferative assay		2 days	Antiproliferative activity against human Caco2 cells after 2 days by sulforhodamine B assay, GI50 = 38.67 μM.	24184076
THP1	Cytotoxicity assay			Cytotoxicity against human THP1 cells by MTT assay, IC50 = 40 μM.	17981462
THP1	Cytotoxicity assay		72 hrs	Cytotoxicity against human THP1 cells after 72 hrs by propidium iodide staining-based flow cytometry, IC50 = 40 μM.	19748781
THP1	Cytotoxicity assay		72 hrs	Cytotoxicity against human THP1 cells assessed as cell viability after 72 hrs by Alamar blue assay, IC50 = 40 μM.	19914747
THP1	Cytotoxicity assay		72 hrs	Cytotoxicity against human THP1 cells using propidium iodide staining after 72 hrs by flow cytometry, IC50 = 40 μM.	20638854
HEK293	Cytotoxicity assay		72 hrs	Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay, IC50 = 40 μM.	28774427
NCH-89	Antiproliferative assay		48 hrs	Antiproliferative activity against human carmustine-resistant NCH-89 cells assessed as BrdU incorporation after 48 hrs, IC50 = 41.2 μM.	20329733
HepG2	Cytotoxicity assay		48 hrs	Cytotoxicity against human HepG2 cells assessed as inhibition of cell growth after 48 hrs by LDH assay, GI50 = 43.09 μM.	25684422
L6	Toxicity assay			Toxicity against rat L6 cells, IC50 = 43.1 μM.	19781948
KB	Cytotoxicity assay		72 hrs	Cytotoxicity against human KB cells after 72 hrs by alamar blue assay, CC50 = 44 μM.	20014857
MKN28	Antiproliferative assay		2 days	Antiproliferative activity against human MKN28 cells after 2 days by sulforhodamine B assay, GI50 = 44.44 μM.	24184076
NFF	Cytotoxicity assay			Cytotoxicity against human NFF cells, IC50 = 46.608 μM.	30245402
J774	Cytotoxicity assay		72 hrs	Cytotoxicity against mouse J774 cells after 72 hrs by CellTiter-96 aqueous one solution cell proliferation assay, EC50 = 47.2 μM.	29215279
HEK293	Cytotoxicity assay		72 hrs	Cytotoxicity against HEK293 cells after 72 hrs by Alamar Blue assay, IC50 = 48 μM.	21190857
MRC5	Cytotoxicity assay			Cytotoxicity against MRC5 cells	17166718
HeLa	Function assay	10 uM	1 hr	Stimulation of reactive oxygen species in human HeLa cells at 10 uM in presence of 10 uM CuCl2 after 1 hr by dihydroethidium dye based fluorescence assay	19748786
A673	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells	29435139
DAOY	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells	29435139
Saos-2	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells	29435139
RD	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for RD cells	29435139
BT-37	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells	29435139
BT-12	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells	29435139
MG 63 (6-TG R)	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for MG 63 (6-TG R) cells	29435139
NB1643	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells	29435139
OHS-50	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells	29435139
SK-N-MC	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells	29435139
LAN-5	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells	29435139
HEK293	Autophagy assay	10 uM	8 hrs	Induction of autophagy in HEK293 cells assessed as increase in p62 level at 10 uM after 8 hrs by immunoblot analysis	28481536
HEK293	Autophagy assay	10 uM	8 hrs	Induction of autophagy in HEK293 cells assessed as increase in LC3-2 level at 10 uM after 8 hrs by immunoblot analysis	28481536
LNCAP	Function assay			Reduction in GFP expression in human LNCAP cells transfected with plasmids co-expressing GFP and shRNAs against REV-ERBbeta followed by compound treatment 24 hrs post-transfection and measured 48 hrs post dose	26135471
SKBR3	Function assay			Reduction in GFP expression in human SKBR3 cells transfected with plasmids co-expressing GFP and shRNAs against REV-ERBbeta followed by compound treatment 24 hrs post-transfection and measured 48 hrs post dose	26135471
HepG2	Function assay			Reduction in GFP expression in human HepG2 cells transfected with plasmids co-expressing GFP and shRNAs against REV-ERBbeta followed by compound treatment 24 hrs post-transfection and measured 48 hrs post dose	26135471
H460	Apoptosis assay	25 to 75 uM	24 hrs	Induction of apoptosis in human H460 cells at 25 to 75 uM after 24 hrs by annexin-V staining-based flow cytometry	25699157
HepG2	Function assay	50 uM	6 hrs	Reduction in lysosomal acidification in human HepG2 cells at 50 uM after 6 hrs by DAPI/lyso tracker red-double staining based confocal microscopic analysis relative to control	28197320
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Informations chimiques, stockage et stabilité

Poids moléculaire	515.86	Formule	C₁₈H₂₆ClN₃.2H₃O₄P	Stockage (À compter de la date de réception)	3 ans-20°Cpoudre
N° CAS	50-63-5	Télécharger le SDF		Stockage des solutions mères	1 an-80°Cen solvant 1 mois-20°Cen solvant
Synonymes	N/A			Smiles	CCN(CC)CCCC(C)NC1=C2C=CC(=CC2=NC=C1)Cl.OP(=O)(O)O.OP(=O)(O)O

Solubilité

In vitro
Lot:

Water : 100 mg/mL

DMSO : Insoluble
(Le DMSO contaminé par lhumidité peut réduire la solubilité. Utiliser du DMSO frais et anhydre.)

Ethanol : Insoluble

Calculateur de molarité

Masse	Concentration	Volume	Poids moléculaire
=	×	×

Calculateur de dilution Calculateur de poids moléculaire

In vivo
Lot:

Calculateur de formulation in vivo (Solution claire)

Étape 1 : Saisir les informations ci-dessous (Recommandé : Un animal supplémentaire pour tenir compte des pertes pendant lexpérience)

Dosage : mg/kg Poids moyen des animaux : g Volume de dosage par animal :μL Nombre danimaux :

Étape 2 : Saisir la formulation in vivo (Ceci est seulement le calculateur, pas la formulation. Veuillez nous contacter dabord sil ny a pas de formulation in vivo dans la section Solubilité.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Résultats du calcul :

Concentration de travail : mg/ml;

Méthode de préparation du liquide maître DMSO : mg médicament prédissous dans μL DMSO ( Concentration du liquide maître mg/mL, Veuillez nous contacter dabord si la concentration dépasse la solubilité du DMSO du lot de médicament. )

Méthode de préparation de la formulation in vivo : Prendre μL DMSO liquide maître, puis ajouterμL PEG300, mélanger et clarifier, puis ajouterμL Tween 80, mélanger et clarifier, puis ajouter μL ddH₂O, mélanger et clarifier.

Méthode de préparation de la formulation in vivo : Prendre μL DMSO liquide maître, puis ajouter μL Huile de maïs, mélanger et clarifier.

Note : 1. Veuillez vous assurer que le liquide est clair avant dajouter le solvant suivant.
2. Assurez-vous dajouter le(s) solvant(s) dans lordre. Vous devez vous assurer que la solution obtenue, lors de lajout précédent, est une solution claire avant de procéder à lajout du solvant suivant. Des méthodes physiques telles que le vortex, les ultrasons ou le bain-marie chaud peuvent être utilisées pour faciliter la dissolution.

Mécanisme daction

Targets/IC₅₀/Ki	TLR3 TLR7 TLR8 TLR9 ATM (Cell-free assay)
In vitro	La Chloroquine est un agent chimiothérapeutique pour le traitement clinique du paludisme. La Chloroquine est capable de se lier à l'ADN et d'inhiber la réplication de l'ADN et la synthèse de l'ARN, ce qui entraîne la mort cellulaire. L'effet de la Chloroquine peut également être lié à la formation d'un complexe toxique hème-Chloroquine. La Chloroquine inhibe la dégradation de l'hémoglobine des trophozoïtes en augmentant le pH vacuolaire et en inhibant l'activité de la phospholipase vacuolaire, des protéases vacuolaires et de l'hème polymérase. La Chloroquine possède des propriétés antirhumatismales définies. La Chloroquine a des effets immunomodulateurs, supprimant la production/libération du facteur de nécrose tumorale et de l'interleukine 6. De plus, la Chloroquine exerce des effets antiviraux directs, inhibant les étapes pH-dépendantes de la réplication de plusieurs virus, y compris les membres des flavivirus, rétrovirus et coronavirus. Ses effets les mieux étudiés sont ceux contre la réplication du VIH. La Chloroquine peut s'accumuler à l'intérieur du phagolysosome macrophagique par piégeage ionique où elle exerce une puissante activité antifongique contre Histoplasma capsulatum et Cryptococcus neoformans par des mécanismes distincts. La Chloroquine inhibe la croissance de H. capsulatum par privation de fer pH-dépendante, tandis qu'elle est directement toxique pour C. neoformans.
In vivo	La Chloroquine a des effets inhibiteurs et promoteurs de tumeurs dans le cancer du sein triple négatif.
Références	[1] https://pubmed.ncbi.nlm.nih.gov/8361993/ [2] https://pubmed.ncbi.nlm.nih.gov/14592603/ [3] https://pubmed.ncbi.nlm.nih.gov/10972492/ [4] https://pubmed.ncbi.nlm.nih.gov/18305095/ [5] https://pubmed.ncbi.nlm.nih.gov/25385822/ [6] https://pubmed.ncbi.nlm.nih.gov/24273604/

Applications

Méthodes	Biomarqueurs	PMID
Western blot	p62 / LC3 ZEBRA / p-S824 KAP1	26677873
Immunofluorescence	SQSTM1 / MAP1LC3B CD1d-β2m / LAMP1 p-S824 KAP1 / ZEBRA	29872540
Growth inhibition assay	Cell viability	25521075

Informations sur lessai clinique

(données du https://clinicaltrials.gov, mis à jour le 2024-05-22)

Numéro NCT	Recrutement	Conditions	Promoteur/Collaborateurs	Date de début	Phases
NCT05841758	Not yet recruiting	Sarcoidosis Pulmonary	Hospices Civils de Lyon	April 1 2024	Phase 4
NCT04443270	Unknown status	COVID-19	CMN 20 de Noviembre	July 27 2020	Phase 1
NCT04462367	Active not recruiting	COVID19\|Coronavirus Infection\|Pregnancy Disease\|Severe Acute Respiratory Syndrome	Instituto Materno Infantil Prof. Fernando Figueira	July 1 2020	--
NCT04340544	Terminated	COVID-19	University Hospital Tuebingen\|Robert Bosch Medical Center\|Universitätsklinikum Hamburg-Eppendorf\|Bernhard Nocht Institute for Tropical Medicine	April 22 2020	Phase 2
NCT04334148	Completed	COVID-19	Adrian Hernandez\|Patient-Centered Outcomes Research Institute\|Duke University	April 22 2020	Phase 3

Support technique

Instructions de manipulation

Tel: +1-832-582-8158 Ext:3

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